Nucleofection as an Efficient Method for Alpha TC1-6 Cell Line Transfection

An efficient transfection is a crucial step for the introduction of epigenetic modification in host cells, and there need an optimized process individual model systems separately. Mouse pancreatic αTC1-6 which act as attractive system cell reprogramming diabetes treatment, were transiently transfected with two different methods: chemical method polyethyleneimine (PEI) nucleofection physical method. Flow cytometry fluorescent microscopy examination GFP expression showed that efficiency was affected by size plasmids using both methods. Subsequently, Cas9 mRNA confirmed successful EpiCRISPR plasmid, whereas physiology remained unchanged. The adjusted protocol cells mix reached 71.1% GFP-positive on fifth post-transfection day proved to be much more than 3.8% PEI cells. Modifying protocol, we finally specify CM-156 program SF 4D-Nucleofector X Solutions Amaxa™ choice alpha TC1-6 line transfection.